Raman
| Voyage™
| Microscope | ||
| Compatible Microscope | Olympus BX51 | Nikon Eclipse 80i |
| Z Adjustment Resolution | 1 μm | |
| X/Y Resolution | Depending on stage and objectives | |
| Depth of Focus | 0.5 μm to 180 μm up to objective | |
| Camera | PGR Chameleon™ USB 2.0 Digital Camera | |
| Lasers | ||
| Excitation | 532 nm excitation | 785 nm excitation |
| Single Mode | ☑ | ☑ |
| Multimode | NA | ☑ |
| Linewidth | < 1 cm-1 | ± 0.02 nm (single), ± 0.2 nm (multimode) |
| Coupling | NA | 9 μm Fiber Single Mode 50 μm Fiber Multimode |
| Wavelength | 532 ± 0.5 nm | 785 ± 0.5 nm |
| Power at Sample | 20 - 22 mW | > 15 mW (single mode) > 150 mW (multimode) |
| Adjustment Power | Neutral density filters at 100%, 79%, 50%, 25%, 10%, 5%, 1% and 0% |
I2C controlled 0-100% adjustable (for multimode laser only) |
| Spectrometer | ||
| Spectral Range | 100 - 3050 cm-1 | 100 - 2550 cm-1 (100 - 3100 cm-1 optional) |
| Spectral Resolution | 3.8 cm-1 | 3 cm-1 (4.5 cm-1 optional) |
| Signal-to-Noise Ratio | Silicon 3rd order Raman peak SNR > 6:1 | |
| Detector | ||
| Detector Type | TE Cooled, Back-thinned, 2D-binning CCD | |
| Detector | Hamamatsu S10141-1107S, TE Cooled | |
| TE Cooling Temperature | -20°C | |
| Maximum Quantum Efficiency | 90% | |
| Number of pixels(2D) | 2048 (H) x 122 (V) | |
| Pixel Size | 12 x 12 μm | |
| Pixel Resolution | 1.75 cm-1 | |
| Adjustable Slit | 0 - 2 mm Adjustable | |
| Digitization Resolution | 16-bit or 65,535:1 | |
| Digitizer Speed | 250 KHz | |
| Integration Time | 12 ms - 120 min | |
| Software | ||
| Software | BWSpec™ | |
| Operation Mode | Linear Mode & 2D Mode | |
| Binning Option (2D Mode) | Odd Row Binning | |
| Data Format | TXT, SPC, and EXCEL | |
| System Operation | ||
| Computer Interface | USB 2.0/1.1 | |
| Power Input | 110-240 VAC, 50-60 Hz | |
| Environment Temperature | 20 - 28°C | |
| Humidity | < 75% RH | |
| Form Factors | |||
| Model | Olympus BX51 | Nikon Eclipse 80i | Retrofit |
| Dimension | 318 x 412 x 415 mm | 300 x 561 x 514 mm | 345 x 128 x 355 mm |
| Weight | 27 kg | 27 kg | 9 kg |
The center wavelength of the laser line is precisely maintained even when the peak power is increased by utilizing a series of high end filters. A laser line filter is used to clean up any side bans and ensure a narrow excitation is delivered to the sample by removing all secondary excitation lines before exciting the sample. The light collected from the sample is then filtered via a notch filter. Finally, an ultra steep long pass filter further removes lingering laser line to allow accurate measurement of Raman peaks as close as 100cm-1 from the Rayleigh line.
The Voyage™ features a back-thinned two dimensional charge coupled device (CCD) to detect the dispersed Raman signal. The CCD is used to obtain 90% QE via collection of incoming photons at wavelengths that would not pass through a front illuminated CCD. The detector is TE cooled to -20°C to maximize dynamic range by reducing dark current. 2D binning operation mode enables maximization of the SNR. As a result the 4th order Raman peak of Si can be detected in less than one minute.
In Raman spectroscopy it is essential to utilize a clean, narrow bandwidth laser due to the fact that the quality of the Raman peaks are directly affected by the sharpness and stability of the delivered light source. The Voyage™ spectrometer system features a patented CleanLaze® technology with linewidth as narrow as 0.02 nm when equipped with our 785 nm laser. This technology results in the correct center wavelength and avoids the phenomenon of “mode hopping.” In addition, the laser output power can be adjusted in the software from 0 - 100%, preventing damage to the sample from laser exposure. Our standard automatic shutter will reduce photobleaching for a variety of different sample types.
Laser lifetime of 10,000 hours ensures quality data for years to come!
B&W Tek’s confocal design is compatible with the two most widely used microscopes: Olympus BX51 and Nikon 80i. For users who already have microscopes, the retrofit (an innovative, confocal sub-assembly) is an extremely cost-effective solution for them to turn their microscopes into confocal Raman microscopes. The retrofit is very simple to install with no alignment work needed.
To achieve the highest degree of confocality, B&W Tek’s confocal design features two optically conjugated pinholes, one of which is placed in the laser path and the other is placed at the entrance of the spectrometer. Therefore, the sample volume from which the light is collected and the amount of out-of-focus light blocked is precisely defined. Such design offers the real and the best confocal performance without any sacrificing efficiency or ease of use.
B&W Tek’s proprietary design of double path transmission optics is a key element in achieving research level spectral resolution of 3 cm-1. The innovative optics makes the combination of high throughput and high resolution possible.
Two Raman probe options are available to allow sampling of various materials in the form of liquids, gels, powder, or solids under lab conditions (Lab Grade) or demanding environmental conditions (Industrial Grade).
| Lab Grade (BAC100) | Industrial Grade (BAC101) | |
| Excitation Fiber | 105μm core w/ FC/PC connector | |
| Collection Fiber | 200nm core with SMA 905 male connector | |
| Fiber Length | 1.5m | |
| Excitation Wavelength | 785nm, 532nm, Other options available | |
| Laser Blocking | OD6 default, OD8 optional | |
| Shaft Material | SS 316L | SS 316L, Hastelloy C-276, Titanium |
| Shaft Diameter | 9.5mm | 12mm |
| Shaft Length | 76.2mm | 76.2mm - 203.2mm (Custom lengths available) |
| Window Material | Quartz | Fused Silica, Sapphire, Sapphire Ball Lens |
| Seal Material | Adhesive sealed | Kalrez® O-ring, Custom |
| Working Distance | 5.90mm | 5mm, 10mm, 20mm |
| Spot Size | 85μm | 85μm |
| Maximum Pressure | 30 PSI | 300 PSI |
| Maximum Temperature | 80°C | 300°C at probe tip |
Our Enhanced Raman Cuvette Holder (BCR100A) is a uniquely designed sampling device that can increase Raman signal up to 3x’s in comparison to a standard cuvette holder by utilizing precise focusing; featuring a three point locking mechanism for reproducibilty. The enhanced Raman cuvette holder can be used with any standard 12.5mm x 12.5mm size cuvette to hold liquid or powder samples.
The BCR100A brings many advantages to Raman measurements; High Stability, Repeatability, Enhanced Raman Signal.
Liquid sample flow cell is a sampling device designed for Raman online process monitoring. It provides a sampling platform with high throughput and stability.
Our Flow Cells are constructed with your choice of three different cell materials, 316 SS, Titanium, or Teflon. Window options include Quartz or Sapphire. The BAC160 uses a Kalrez® O-ring which creates a chemical resistant sampling device.
Custom cell material and window construction available upon request.
| Cell Material | 316 SS or Titanium |
| Fitting | 0.5in Swagelok |
| Tubing OD | 0.5in – 2in |
| O-ring Material | Kalrez® O-ring Other materials on request |
| Window Material | Quartz Sapphire Other materials on request |
| Working Distance | Fixed |
| Excitation Wavelength Range | 300nm – 2500nm |
| Maximum Temperature | 150°C |
| Maximum Pressure | 150 PSI |
| Weight | 0.5 lbs |
With a small laser spot size of 85mm, laser focus at the sample area becomes critical in all dimensions. The BAC150 precision probe mount accessory provides coarse and fine adjustment knobs for the X, Y, and Z axes. Z-axis adjustment allows for laser focusing on the desired plane in order to maximize the Raman signal.
The BAC151 is a visual assisted sampling platform that utilizes video precision spot sampling at a fraction of the cost of a typical Raman Microscope. The digital camera and LED illuminator allows for precision targeting and focusing of the spot size by way of an on screen display via BWSpec™ software or an added external monitor. Standard objectives from 10x’s - 100x’s can be utilized and sample images can be easily recorded and saved. Both coarse and fine adjustment knobs are available for X, Y, and Z-axis control. Bright and dark field illumination optimizes the light required for various sample surfaces. Compatible with our Lab and Industiral Grade Raman probes, this video microscope system provides advantages of Raman microscopy at a fraction of the cost compared to similar research instruments.
Thermo Scientific GRAMS/AI® Lab software package: for processing and managing spectroscopy data
PLSplus/IQ for Thermo Scientific GRAMS/AI 8: for creating qualitative and quantitative chemometric calibration models
Spectral ID for Thermo Scientific GRAMS/AI 8: for qualitative material identification through spectral library search
The foundation for all B&W Tek, Inc. software platforms, BWSpec™ is ideal for broad Raman applications.
BWSpec™ is a spectral data acquisition software developed by B&W Tek, Inc. for use with our spectrometers, systems, and accessories. It is included with the purchase of any product which uses it to operate. The software delivers a wide range of features designed to perform complex measurements and calculations at the click of a button. BWSpec™ features multiple data formats and the capability to optimize scanning parameters such as integration time and laser output power control. In addition to data acquisition and data processing, other features include automatic dark removal, spectrum smoothing, and manual/auto baseline correction. The software also contains an OCX interface for users to collect spectra in Thermo Scientific GRAMS/AI®.
Built on the proven BWSpec™ platform, BWID™ is optimized for identification and verification of materials
BWID™ has been specifically designed for Raman spectroscopy. This software will rapidly identify and verify materials stored in your own personal easy-to-create library or easy-to-load third-party library. One click is all you need to create or expand a Raman library. The combination of BWID™ and any of B&W Tek’s Raman spectrometer systems unite to create a powerful and effective solution for identification and verification of materials. The reporting capability enables a user to save, view, and print any analysis report.
For industrial Raman applications that require federal compliance: BWIDTM-Pharma supports FDA 21 CFR Part 11 Compliance
BWID™-PHARMA is designed for pharmaceutical manufacturing facilities that are facing the increasing need for 100% inspection of incoming raw materials. In addition to all the features provided in BWID™, BWID™-PHARMA provides enhanced system access security and an audit trail of data activities that support compliance with the FDA 21 CFR Part 11 regulation for Electronic Records and Electronic Signatures, benefiting pharmaceutical system validations including Installation Qualifications (IQs) and Operational Qualifications (OQs) procedures.
True Confocality and True Flexibility
The Voyage™ is a Confocal Raman Microscope System that is designed to deliver true confocality and true flexibility. Its robust opto-mechanical design provides one design to fit multiple microscopes, including Olympus BX51 research microscope or Nikon Eclipse 80i advanced research microscope. In addition, the innovative design of the confocal sub-assembly provides the retrofit for users to turn their own microscopes into confocal Raman microscope. Its optimized optical design for confocal concept, combined with B&W Tek’s patented CleanLaze® laser and patent pending high resolution spectrometer, delivers the smallest spot size in the market and a high spectral resolution of 3 cm-1.
